The study was undertaken to obtain indirect values of volume of packed red cell (VPRC) without centrifugation, using the erythrocyte sedimentation rate (ESR) of diluted blood in dogs. ESRs of diluted blood using the diluent of autologous plasma in which formed numerous RBC-rouleau clumps, autologous serum in which formed a few RBC-rouleau clumps, and 5% dextrose or 6% sucrose solutions in which formed numerous RBC-aggregation clumps, were accelerated. But, ESR of diluted blood using the 0.9% saline, D-S, ACD-B, CDP or D-PBS solutions were sluggish, because erythrocytes were dispersed in these diluents.
Reliable values of VPRC on the basis of the correlating regressive equation to the ESR could be derived from values of 60˚-angled-micro-ESR/40 min in the mixture, four parts of 5% dextrose solution and one part of whole blood.
In the ESR values of diluted blood with low ratio, 1 : 1∼3 : 1, 60˚-micro-ESR was higher than 60˚-Wintrobe-ESR. But, in the diluted blood with high ratio, 4 : 1∼5 : 1, there was no different ESR values. For an aid of practical use, authers suggested a list of the 60˚-micro-ESR/40 min in the diluted blood with equivalent VPRC of whole blood.
|